The present study attempts to re-examine the concept of well-being at work in the contemporary scenario. The nature of work and workplace are changing rapidly, increased competition, work pressure, 24X7 work culture, etc. has made it important to re-examine the current literature on well-being at work to address the growing issues of stress, anxiety, and work-life imbalances. An exploratory study was also undertaken with the aim to understand the perspectives that prevail amongst the working professionals concerning the concept of well-being at work. Based on literature and results of the exploratory study, the paper attempts to re-define the concept of well-being at work by collectively examining all of its dimensions, viz. psychological, physical, financial, social, career and spiritual well-being, to make it more holistic. In the extant literature the dimension of spiritual well-being has been studied separately, and never been integrated with the other dimensions of well-being. The present paper proposes a more holistic framework on well-being at work which considers all the dimensions collectively in the work context.